Identifikasi Gen SHV pada Enterobacteriaceae Produsen Extended-Spectrum Beta-Lactamases (ESBLs)

Yuwono Yuwono

Abstract


Salah satu problem resistensi terhadap antimikroba terutama di rumah sakit adalah kelompok
Enterobacteriaceae yang memproduksi Extended-Spectrum Beta-Lactamases (ESBLs). Tujuan
penelitian ini adalah untuk mengidentifikasi gen SHV pada Enterobacteriaceae produsen ESBLs pada
pasien yang dirawat di RSUP Mohammad Hoesin Palembang. Didapatkan 75 sampel
Enterobacteriaceae berdasarkan uji Double Disk Approximation dan PCR. Spesies yang ditemukan
adalah Klebsiella pneumoniae 30 (40,00%), Escherichia coli 17 (22,66%), Enterobacter sp 14
(18,67%), Proteus Sp 12 (16,00%), dan Acinetobacter calcoaceticus 2 (2,67%). Berdasarkan hasil
PCR didapatkan 32 sampel mengandung gen SHV. Distribusi gen SHV adalah 20 (62,50%) pada
Klebsiella pneumoniae, 7 (21,87%) pada Escherichia coli, 4 (12,50%) pada Enterobacter sp dan 1
(3,13%) pada Proteus sp. Hasil penelitian ini menunjukkan bahwa Klebsiella pneumoniae dominan
pada Enterobacteriaceae produsen ESBLs dan gen SHV juga dominan ditemukan pada spesies ini.


Keywords


ESBL, Enterobacteriaceae, gen SHV

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DOI: https://doi.org/10.32502/sm.v4i1.1413

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