Infeksi Chlamydia Trachomatis pada Penderita Karsinoma Serviks Terinfeksi Human Papilloma Virus (HPV) tipe 16 di RSUP Dr. Sardjito Yogyakarta

Indri Ramayanti

doi:10.32502/sm.v1i1.44

Infeksi Chlamydia Trachomatis pada Penderita Karsinoma Serviks Terinfeksi Human Papilloma Virus (HPV) tipe 16 di RSUP Dr. Sardjito Yogyakarta

Indri Ramayanti

Abstract

Cervical carcinoma is one of the most common malignant neoplasm amongwomen
in developing countries and remains as a major health problem world wide. In Indonesia,
the incident of cervical carcinoma are around 180.000 new cases per year or 90-100 nevv
cancers among 100.000 communities. Several studies have reported the role of HPV as an
STD agent (Sexually Trasmitted Disease). Cofactor in the HPV infection accelerate up the
development of infection into cancer One of the STD cofactor is co infection with C.
trachomatis. Infection ofC. trachomatis is perceived as cofoctor that synergatedwith HPV
throughfree radical mechanism and immune system.
The objective of the study is to know the presence of C. trachomatis infection had
potencyfor being cofactor HPV I 6 in cervical carcinoma patients.
Twentylhree sample of cervical smeati cervical carcinoma biopsies and fresh
tissue obtained from surgerydep, from DR. Sardjito Hospital, Yogtalurta. The DNA
isolated of samples were amplified by PCR using primer (E6for-vvard and reversed) from
ORF E6 HPV I 6. Sample with positive DNA HPV I 6 was then ampli/ied C. trachomatis gen
us i ng p ri m er (C C 3 / C C Q from th e s e q uen c e of cry ptic C hl amy dia p I asmid.
The research showed thatfrom 23 cases of cervical carcinomawere obtained HPV
positive type 16 with 16 cases (69,5%o). From 16 cases of HPV type 16 positive cervical
carcinoma, 15 cases were examined to know the presence of C. trachomatis and were
obtained 8 cases (53,j%o) as C. trachomatis positive.

Keywords

Cervical Carcinoma, HPV 16, Chlamydia Trachomatis, Cofactor

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DOI: https://doi.org/10.32502/sm.v1i1.44